Our Steam-Thru connector can be mounted into an assembly and be sterilized by gamma irradiation. The assembly with the Steam-Thru connector can be installed at the vessel by Tri-Clamp connection. The non-sterile connection between the Tri-Clamps will be treated with steam. Then the valve in the Steam-Thru connector can be activated and the flow path between the vessel and the single-use assembly is open.